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1.
Arthrosc Tech ; 13(2): 102861, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38435251

RESUMEN

Terrible triad injuries are typically treated surgically to restore elbow stability, as the radial head acts as a secondary stabilizer to valgus stress, while the coronoid provides stability against posterior elbow dislocations. The lateral ulnar collateral ligament (LUCL) is also commonly injured in terrible triad of the elbow injuries, and if not repaired, leads to posterolateral rotatory instability. Depending on the fracture pattern and size, the radial head fracture may be treated with open reduction internal fixation (ORIF), arthroplasty, or excision, whereas the coronoid fracture is most commonly treated with ORIF. If treated, these injuries are managed prior to LUCL fixation to avoid stressing the LUCL repair. We describe a technique for treatment of a LUCL injury with a suture button. When repairing the LUCL, a Kocher approach is used to visualize the LUCL footprint, which is then reattached to the insertion point on the lateral epicondyle using a suture button. The purpose of this study was to provide a step-by-step approach to using this surgical technique and an associated postoperative protocol.

2.
Orthop J Sports Med ; 11(6): 23259671231164670, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37347024

RESUMEN

Background: Increased posterior tibial slope (PTS) is a risk factor for knee pathology. Accurate measurement of PTS is predicated on a quality lateral knee radiograph; however, little is known about how the quality of the radiograph affects the measured PTS. Purposes: To (1) describe a method for measuring malalignment on lateral knee radiographs, (2) assess the effects of malpositioning of the knee on radiographic measures of malalignment, and (3) determine any correlations between malalignment and the measured PTS. Study Design: Descriptive laboratory study. Methods: Using a setup similar to that of a standard radiology suite, 25 sets of radiographs were taken using 5 sawbone models. Each set included a true lateral view and separate malpositioned radiographs at 5°, 10°, and 15° of adduction, abduction, internal rotation, and external rotation. Malalignment for each radiograph was quantified as the anterior-posterior distance (APD) and proximal-distal distance (PDD) between femoral condyles. The medial PTS was measured in duplicate, and the interrater reliability was calculated. Results: The interrater reliability was excellent, with intraclass correlation coefficients of 0.92, 0.91, and 0.96 for the APD, PDD, and PTS, respectively. Malrotation significantly affected the APD (P < .001), with a mean change of 5.6 mm per 5°. Malpositioning in abduction/adduction significantly affected the PDD (P < .001), with a mean change of 5.1 mm per 5°. There was no significant impact of rotation or APD on the PTS. Abduction/adduction did affect the PTS (P < .001) above a threshold of 5° of malpositioning. The PTS decreased as the PDD increased, moving from adduction to abduction (R2 = 0.5687). Conclusion: The measured PTS was more sensitive to malpositioning by abduction/adduction than by malrotation. Malrotation affected the APD, while abduction/adduction affected the PDD. Thus, the accuracy of the measured PTS was compromised more by poorly aligned distal femoral condyles than it was by poorly aligned posterior femoral condyles. Clinical Relevance: To minimize the effects of malpositioning, we recommend utilizing radiographs with a |PDD| of <5 mm and an |APD| of <15 mm when measuring the PTS.

3.
Phys Sportsmed ; 51(5): 414-419, 2023 10.
Artículo en Inglés | MEDLINE | ID: mdl-35583477

RESUMEN

Ice hockey is a fast-paced contact sport with a high rate of injury. While many of the injuries are acute and related to high skating speeds, frequent collisions, and sharp skates, the clinician must also be aware of the chronic injuries that commonly arise from playing this sport. The "Bauer bump" is one such chronic injury, which is the onset of Haglund syndrome in ice hockey players occurring in the context of wearing ice hockey skates. With this condition, players notice a bony enlargement of their posterosuperior calcaneus with or without the accompanying symptoms of retrocalcaneal bursitis and insertional Achilles tendinopathy. It is important for clinicians to understand the nature of Haglund syndrome in hockey players so that it can be appropriately diagnosed, managed, and ultimately, prevented.


Asunto(s)
Tendón Calcáneo , Calcáneo , Hockey , Tendinopatía , Humanos , Hockey/lesiones , Síndrome
4.
Hand (N Y) ; 18(1): 133-138, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-33789496

RESUMEN

BACKGROUND: This study directly compares the recurrence rates of dorsal wrist ganglion cysts in patients treated via open surgical excision versus arthroscopic surgical excision. We hypothesized that there would be no difference between recurrence rates with these 2 surgical options. METHODS: We retrospectively reviewed the charts of all patients with a dorsal ganglion cyst undergoing either open or arthroscopic surgical excision at a single academic center with 3 fellowship-trained attending hand surgeons from 2012 to 2017. Charts were identified using Current Procedural Terminology codes and were reviewed using postoperative office notes for preoperative and postoperative symptoms, episodes of recurrence, time at which recurrence occurred, subsequent operations, and outcome at final follow-up. RESULTS: The charts of 172 patients undergoing either arthroscopic or open dorsal ganglion excision were reviewed. Nine of 54 (16.7%) arthroscopic excisions resulted in cyst recurrence, while 8 of 118 (6.8%) open excisions resulted in cyst recurrence (P = .044). Two of 9 (22%) recurrences after arthroscopic ganglion excision versus 2 of 8 (25%) recurrences after open ganglion excision underwent repeat surgical intervention. Time to recurrence, as well as final follow-up, was not statistically different between groups. CONCLUSIONS: Dorsal wrist ganglion cysts are the most common benign soft tissue mass of the upper extremity, but it remains unknown whether arthroscopic or open surgical excision leads to lower recurrence rate. Scant literature exists directly comparing these 2 methods of surgical excision. This study suggests that open excision of dorsal wrist ganglia leads to a lower recurrence rate than does arthroscopic excision.


Asunto(s)
Ganglión , Muñeca , Humanos , Muñeca/cirugía , Ganglión/cirugía , Estudios Retrospectivos , Articulación de la Muñeca/cirugía , Artroscopía/métodos
5.
J Surg Orthop Adv ; 32(4): 232-237, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-38551230

RESUMEN

The purpose of this study was to compare mortality and complication rates among geriatric patients who sustained a hip fracture before the coronavirus disease (COVID-19) pandemic began to those who presented during the peak of the pandemic. Patients greater than or equal to 50 years of age who presented with a diagnosis of hip fracture from March 16, 2020 to May 1, 2020 were compared with a historical control group who presented between December 1, 2019 and January 16, 2020. Minimum follow up was 30 days. The primary outcome was a 30-day mortality rate. Thirty-day mortality was significantly different between groups, with no deaths of 24 patients in the pre-COVID-19 cohort versus six deaths of 23 patients (26.1%) in the COVID-19 cohort (chi-squared test, p-value = 0.02). The study concluded that the COVID-19 pandemic increased mortality risk for geriatric hip fracture patients. (Journal of Surgical Orthopaedic Advances 32(4):232-237, 2023).


Asunto(s)
COVID-19 , Fracturas de Cadera , Humanos , Estados Unidos/epidemiología , Anciano , COVID-19/epidemiología , COVID-19/complicaciones , Pandemias , Estudios de Seguimiento , Fracturas de Cadera/cirugía , Hospitales , Estudios Retrospectivos
6.
J Wrist Surg ; 12(6): 534-539, 2023 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-38213563

RESUMEN

Background Volar locking plate fixation (VLP) is commonly used to treat distal radius fractures (DRF). Risk of dorsal compartment injury with distal screw hole fixation has been studied; however, the risk with proximal screw hole fixation is not well studied. Purpose The goal of this study was to investigate the risk of dorsal structure injury from the screw holes proximal to the two distal rows. Methods Nine cadaveric forearms were used. After volar distal radius exposure, a long VLP was applied. Kirschner wires were placed through the most proximal holes into the dorsal compartments. The extensor structures penetrated were noted and tagged with hemoclips. The distance from the dorsal cortex to the structures was measured. Results The abductor pollicis longus (APL) and extensor pollicis brevis (EPB) muscle bodies were only penetrated; no tendons were penetrated. Proportion of muscle penetration increased with the more proximal screw holes. EPB was more likely to be penetrated distally and APL proximally; both were injured at holes 2 and 3. The extensors were 2 mm from the dorsal cortex of the radius on average; this did not decrease with compression of the forearm. Conclusions This is the first study to examine the anatomic risk of extensor structure injury with VLP proximal screw hole penetration. No extensor tendons were penetrated by these proximal screw holes; first dorsal compartment muscle bellies may be irritated with overpenetration. Our findings suggest that proximal VLP screws do not need to be downsized if they are not over 2 mm prominent.

7.
Front Sports Act Living ; 4: 890429, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35847454

RESUMEN

Background: Though once considered an integral part of professional hockey, fighting carries significant health risks to players. Fighting has remained legal in the National Hockey League (NHL) due to its purported economic and entertainment value. However, fights per game have diminished over the past 20 years, challenging the necessity of fighting to promote fan attendance. Hypothesis: Despite decreasing fighting rates, attendance has been stable and is negatively associated with fights per game. Methods: Two public databases were reviewed to determine attendance, fighting majors, goals scored, and games played for each NHL team from 2000 to 2020 and averaged on a per game basis. Univariate analysis was used to evaluate relationships between attendance and fights, attendance and goals, as well as goals and fights. Results: Fights per game decreased from a peak of 0.64 in 2002 to a low of 0.18 in 2020, while average attendance increased from a low of 16,549 in 2004 to a peak of 17,768 in 2013, before settling between 17,400 and 17,500 during the final three seasons of the study period. A significant negative correlation was found between attendance and fights per game (R = -0.6617, p = 0.0020). There was a positive, but not significant correlation between attendance and goals per game (R = 0.2457, p = 0.3105). A significant inverse correlation existed between goals per game and fights per game (R = -0.521, p = 0.0222). Conclusions: NHL fighting rates have diminished during the past two decades, while fan attendance has increased. A significant negative correlation exists between fan attendance and fights per game, casting doubt on fighting's entertainment value. Meanwhile, a significant inverse correlation was noted between goals per game and fights per game. Taken together, these findings suggest fans may prefer higher scoring and less violent competitions. We conclude by suggesting that prohibiting fights in the NHL could improve player safety without negatively impacting fan attendance.

8.
Cartilage ; 13(3): 19476035221098164, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35819020

RESUMEN

OBJECTIVE: The objective of this study was to identify and describe the existing literature on criteria used for return to play (RTP) following surgical management of osteochondral defects of the knee. DESIGN: A systematic review was performed to evaluate the surgical management of osteochondral defects of the knee in skeletally mature patients with a minimum of 2-year follow-up using Level I to IV studies in PubMed EMBASE from January 1998 to January 2016. RESULTS: Twelve studies with at least one explicitly stated criterion for RTP were identified from a review of 253 published articles. The majority of included studies were Levels II and IV (33%, respectively). Autologous chondrocyte implantation (ACI) was exclusively evaluated in 33.3% of papers and 16.7% evaluated osteochondral allograft transplantation (OCA). Eight different RTP criteria were used alone or in combination across the reviewed studies and time was the most often utilized criterion (83.3%). Minimum time to RTP ranged from 3 to 18 months. CONCLUSIONS: This systematic review identifies current criteria used in the available literature to dictate RTP. Time from surgery was the most commonly employed criterion across the reviewed studies. Given the complex biological processes inherent to the healing of cartilaginous defects, further research is needed to design more comprehensive guidelines for RTP that are patient-centered and utilize multiple functional and psychological domains relevant to the process of returning to sport.


Asunto(s)
Enfermedades de los Cartílagos , Cartílago Articular , Fracturas Intraarticulares , Enfermedades de los Cartílagos/cirugía , Cartílago Articular/cirugía , Humanos , Rodilla , Articulación de la Rodilla/cirugía , Volver al Deporte
9.
Hand (N Y) ; 17(1_suppl): 87S-94S, 2022 12.
Artículo en Inglés | MEDLINE | ID: mdl-35168382

RESUMEN

Distal radius fractures are common orthopedic injuries. Treatment has varied historically, but volar locking plating currently predominates. Although flexor tendon injury is a well-studied complication of this operation, extensor tendon injury is less well studied. The purpose of this review is to search the literature and present the epidemiology, presentation, and treatment of this complication. The Cochrane, EMBASE, PubMed, and SCOPUS databases were searched for the terms "volar" + "radius" + ("plate" OR "plating") + "extensor." Ninety final studies were included for analysis in this review. The incidence of extensor tendon rupture varies from 0% to 12.5%; the extensor pollicis longus is most commonly ruptured. The presentation and management of extensor tendon injury after injury, intraoperatively, and postoperatively are summarized. Radiographic views are described to detect screw prominence and minimize intraoperative risk. Extensor tendon injury after volar locking plate for distal radius fractures is an uncommon injury with several risk factors including dorsal screw prominence and fracture fragments. Removal of hardware and tendon transfers or reconstruction may be necessary to prevent loss of extensor mechanism.


Asunto(s)
Fracturas del Radio , Traumatismos de los Tendones , Fracturas de la Muñeca , Humanos , Fracturas del Radio/diagnóstico por imagen , Fracturas del Radio/cirugía , Fracturas del Radio/complicaciones , Fijación Interna de Fracturas/efectos adversos , Traumatismos de los Tendones/diagnóstico por imagen , Traumatismos de los Tendones/etiología , Traumatismos de los Tendones/cirugía , Rotura/etiología , Rotura/cirugía , Tendones
10.
Hand (N Y) ; 17(1): 35-37, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-32100567

RESUMEN

Background: This cadaveric study defines the interval distance between the proximal insertion of the volar wrist ligaments and the distal edge of the pronator quadratus on the distal radius. It is important to be aware of this distance during surgical dissection for placement of volar locking plates for wrist fractures. Disruption of the volar wrist ligament insertion may have adverse biomechanical consequences such as carpal instability, which can lead to pain and eventually wrist arthritis. Methods: Thirteen cadaveric wrists were dissected using the trans-flexor carpi radialis volar approach to identify relevant anatomy. The distance between the distal border of the pronator quadratus and the most proximal insertion of the volar wrist ligaments was measured. Results: The average distance between the pronator quadratus and the proximal insertion of the volar wrist ligaments was 5 mm, with a standard deviation of 2 mm. Conclusions: The volar wrist ligaments insert quite near the distal end of the pronator quadratus. Surgeons should be cognizant of the proximity of the volar wrist ligaments and be judicious with subperiosteal stripping of the distal fragment during volar plating procedures.


Asunto(s)
Fracturas del Radio , Muñeca , Placas Óseas , Fijación Interna de Fracturas/métodos , Humanos , Ligamentos Articulares/cirugía , Fracturas del Radio/cirugía
11.
Artículo en Inglés | MEDLINE | ID: mdl-34807889

RESUMEN

INTRODUCTION: The Fragility Index (FI) and the Fragility Quotient (FQ) are powerful statistical tools that can aid clinicians in assessing clinical trial results. The purpose of this study was to use the FI and FQ to evaluate the statistical robustness of widely cited surgical clinical trials in orthopaedic trauma. METHODS: We performed a PubMed search for orthopaedic trauma clinical trials in high-impact orthopaedics-focused journals and calculated the FI and FQ for all identified dichotomous, categorical outcomes. RESULTS: We identified 128 studies with 545 outcomes. The median FI was 5, and the median FQ was 0.0482. For statistically significant and not statistically significant outcomes, the median FIs were 3 and 5, and the mean FQs were 0.0323 and 0.0526, respectively. The FI was greater than the number of patients lost to follow-up in most outcomes. CONCLUSIONS: The orthopaedic trauma literature is of equal or higher quality than research in other orthopaedic subspecialties, suggesting that other orthopaedic subspecialties may benefit from modeling their clinical trials after those in orthopaedic trauma.


Asunto(s)
Procedimientos Ortopédicos , Ortopedia , Humanos
12.
J Shoulder Elbow Surg ; 30(8): 1787-1793, 2021 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-33271323

RESUMEN

BACKGROUND: The P value is a statistical tool used to assess the statistical significance of clinical trial outcomes in orthopedic surgery. However, the P value does not evaluate research quality or clinical significance. The Fragility Index (FI) is an alternative statistical method that can be used to assess the quality and significance of clinical research and is defined as the number of patients in a study intervention group necessary to convert an outcome from statistically significant to statistically insignificant or vice versa. The primary purpose of this study was to evaluate the statistical robustness of clinical trials regarding shoulder arthroplasty using the FI. The secondary goal was to identify trial characteristics associated greater statistical fragility. METHODS: A systematic review of randomized clinical trials in shoulder arthroplasty was performed. The FI was calculated for all dichotomous, categorical study outcomes discussed in the identified studies. Descriptive statistics and the Pearson correlation coefficient were used to evaluate all studies and characterize associations between study variables. RESULTS: A total of 13 randomized controlled trials were identified and evaluated; these trials had a median sample size of 47 patients (mean, 54 patients; range, 26-102 patients) and a median of 7 patients (mean, 5.8 patients; range, 0-14 patients) lost to follow-up. The median FI was 6 (mean, 5; range, 1-11), a higher FI than what has been observed in other orthopedic subspecialties. However, the majority of outcomes (74.4%) had an FI that was less than the number of patients lost to follow-up, and most outcomes (89.7%) were statistically insignificant. CONCLUSION: Randomized controlled trials in shoulder arthroplasty have comparable statistical robustness to the literature in other orthopedic surgical subspecialties. We believe that the inclusion of the FI in future comparative studies in the shoulder arthroplasty literature will allow surgeons to better assess the statistical robustness of future research.


Asunto(s)
Artroplastía de Reemplazo de Hombro , Procedimientos Ortopédicos , Ortopedia , Humanos , Ensayos Clínicos Controlados Aleatorios como Asunto , Tamaño de la Muestra
13.
JSES Int ; 4(4): 786-791, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-33345216

RESUMEN

BACKGROUND: The shoulder is a commonly injured area in hockey, yet information is lacking on the prevalence and effect of shoulder instability. Our study investigates the incidence of shoulder dislocation events in the National Hockey League (NHL) and the effects on return-to-play (RTP) and player performance. We hypothesize that NHL players would have high RTP rates without significant changes in performance after injury. METHODS: NHL players who suffered in-season shoulder instability events between 2003-2004 and 2017-2018 seasons were identified. Demographic characteristics, incidence of injury, recurrences, RTP, and statistical performance data were collected. Postinjury performance was compared with experience-matched, era-matched, position-matched, and age-matched controls. A mixed generalized linear regression model was used to compare postinjury performance as a function of operative and nonoperative treatment. RESULTS: A total of 57 players experienced 67 shoulder instability events with 98.5% of players returning to play after an average of 26.3 ± 20.8 regular season games missed. Surgery was performed in 47.8% of players with no recurrent injuries postoperatively. Nonoperatively managed players experienced a decrease in points per game (P = .034) compared with surgically treated patients. Recurrence occurred in 14.3% of conservatively managed players, with 33.3% experiencing a season-ending injury. Players with recurrent injuries missed significantly more career games compared with those treated initially with surgery (P = .00324). CONCLUSION: Professional hockey players experience high rates of RTP with acceptable performance outcomes after shoulder instability events; however, recurrent injuries led to significantly more career games missed when treated nonoperatively at the time of injury.

14.
J Orthop Trauma ; 34(9): e330-e335, 2020 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-32815847

RESUMEN

Although elective surgeries and in-person office visits were greatly reduced during the COVID-19 crisis, orthopaedic surgeons continue to play a critical role in caring for both orthopaedic and nonorthopaedic problems during this pandemic. Orthopaedic departments provide the ability to off-load emergency departments of orthopaedic issues, redeploy staff to areas of need across the hospital system, and provide direct care to COVID-19 patients. The following will discuss the experience of a large academic orthopaedic surgery department within the epicenter of the COVID-19 pandemic with respect to redeployment of human capital and unique resources such as the United States Naval Ship Comfort as well as our recommended strategy for handling future disaster situations.


Asunto(s)
Betacoronavirus , Infecciones por Coronavirus/prevención & control , Servicios Médicos de Urgencia/organización & administración , Personal Militar , Ortopedia/organización & administración , Pandemias/prevención & control , Neumonía Viral/prevención & control , Navíos , COVID-19 , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/epidemiología , Humanos , Ciudad de Nueva York/epidemiología , Neumonía Viral/diagnóstico , Neumonía Viral/epidemiología , SARS-CoV-2
15.
J Shoulder Elbow Surg ; 29(7): 1380-1386, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32553438

RESUMEN

HYPOTHESIS: Heterotopic ossification (HO) is a common complication of surgically treated elbow fractures that can inhibit range of motion and impair quality of life. Although there are many treatment methods for HO, there is a lack of consensus as to the best option. We hypothesized that contracture release combined with Botox injection would lead to improved functional outcome scores when compared with current treatment methods. METHODS: A retrospective review was conducted of patients who presented to a single surgeon with HO secondary to elbow fracture between 2005 and 2018. A total of 59 patients were identified who met inclusion criteria. Data were classified into 3 groups: contracture release (control - CR), Botox injection with CR (Botox + CR), and radiation therapy with CR (CR + RT). Range of motion measurements were obtained, including flexion, extension, pronation, and supination. RESULTS: A total of 30 patients (30 of 59, 50.8%) received CR, 6 (6 of 59, 9.2%) were treated with CR + RT, and 23 (23 of 59, 40.0%) had CR + Botox. There was a significant difference between pre- and postoperative arc of motion for both CR + RT (P < .01) and CR + Botox (P < .01). In addition, there was a significant difference in pre- and postoperative extension for patients who received intraoperative Botox injections (P < .05). There was no significant difference between pre- and postoperative motion nor extension in the CR group. CONCLUSION: Intraoperative Botox injection with CR is an effective method in the treatment of post-traumatic elbow stiffness caused by HO.


Asunto(s)
Toxinas Botulínicas Tipo A/uso terapéutico , Articulación del Codo/cirugía , Fracturas Óseas/cirugía , Liberación de la Cápsula Articular , Fármacos Neuromusculares/uso terapéutico , Osificación Heterotópica/terapia , Adulto , Terapia Combinada , Contractura/etiología , Contractura/terapia , Codo/cirugía , Articulación del Codo/fisiopatología , Femenino , Fijación Interna de Fracturas/efectos adversos , Fracturas Óseas/complicaciones , Humanos , Masculino , Persona de Mediana Edad , Osificación Heterotópica/etiología , Radioterapia , Rango del Movimiento Articular , Estudios Retrospectivos , Lesiones de Codo
16.
Open Access J Sports Med ; 11: 93-103, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32425621

RESUMEN

Ice hockey continues to be a popular, fast-paced, contact sport enjoyed internationally. Due to the physicality of the game, players are at a higher risk of injury. In the 2010 Winter Olympics, men's ice hockey had the highest injury rate compared to any other sport. In this review, we present a comprehensive analysis of evaluation and management strategies of common hand, wrist, and elbow injuries in ice hockey players. Future reseach focusing on the incidence and outcomes of these hand, wrist and elbow injuries in ice hockey players is warranted.

17.
J Cell Sci ; 130(20): 3481-3495, 2017 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-28851806

RESUMEN

Scarring and fibrotic disease result from the persistence of myofibroblasts characterized by high surface expression of αv integrins and subsequent activation of the transforming growth factor ß (TGFß) proteins; however, the mechanism controlling their surface abundance is unknown. Genetic screening revealed that human primary stromal corneal myofibroblasts overexpress a subset of deubiquitylating enzymes (DUBs), which remove ubiquitin from proteins, preventing degradation. Silencing of the DUB USP10 induces a buildup of ubiquitin on integrins ß1 and ß5 in cell lysates, whereas recombinant USP10 removes ubiquitin from these integrin subunits. Correspondingly, the loss and gain of USP10 decreases and increases, respectively, αv/ß1/ß5 protein levels, without altering gene expression. Consequently, endogenous TGFß is activated and the fibrotic markers alpha-smooth muscle actin (α-SMA) and cellular fibronectin (FN-EDA) are induced. Blocking either TGFß signaling or cell-surface αv integrins after USP10 overexpression prevents or reduces fibrotic marker expression. Finally, silencing of USP10 in an ex vivo cornea organ culture model prevents the induction of fibrotic markers and promotes regenerative healing. This novel mechanism puts DUB expression at the head of a cascade regulating integrin abundance and suggests USP10 as a novel antifibrotic target.


Asunto(s)
Cadenas beta de Integrinas/metabolismo , Integrina beta1/metabolismo , Ubiquitina Tiolesterasa/fisiología , Ubiquitinación , Animales , Células Cultivadas , Células HEK293 , Humanos , Proteolisis , Transducción de Señal , Sus scrofa , Técnicas de Cultivo de Tejidos , Factor de Crecimiento Transformador beta/fisiología , Cicatrización de Heridas
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